OECD 219: Sediment-water chironomid toxicity (spiked water)
The OECD 219: Sediment-water Chironomid Toxicity Using Spiked Water Study assesses the effect of a chemical on sediment-dwelling chironomus (midge) larvae. Larvae are deposited into separate sediment-water systems, and the water is spiked with a test chemical. Larval emergence, development and survival are observed for up to 28 days (C. riparius, C. yoshimatsui) or 65 days (C. tentans). Statistical regressions are then run to determine the relationship between a chemical and any reduction in chironomid emergence and development.